Two-Step Activation Mechanism of the ClpB Disaggregase for Sequential Substrate Threading by the Main ATPase Motor
Summary: AAA+ proteins form asymmetric Generations hexameric rings that hydrolyze ATP and thread substrate proteins through a central channel via mobile substrate-binding pore loops.Understanding how ATPase and threading activities are regulated and intertwined is key to understanding the AAA+ protein mechanism.We studied the disaggregase ClpB, whi